Biochemistry in the Lab

A Manual for Undergraduates

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. Les mer
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Innbundet
Legg i
Vår pris: 1983,-

(Innbundet) Fri frakt!
Leveringstid: Sendes innen 21 dager
På grunn av Brexit-tilpasninger og tiltak for å begrense covid-19 kan det dessverre oppstå forsinket levering.

Om boka

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.


Key Features:








Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience







Includes chemical biology as its foundation, teaching readers experimental methods specific to the field







Provides instructor experiments that are easy to prepare and execute, at comparatively low cost







Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry







Is written by an expert in the field





This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Fakta

Innholdsfortegnelse

Buffers


Assays


ELISA


Protein Concentration


Salting out Proteins and other Biomolecules


A Discussion of Isoelectric Point and Effective Charge


Column Chromatography


Michaelis-Menten Kinetics


Protein Purification


Polyacrylamide Gels


in silico Biochemistry: the evolution of globins


Growing Crystals of Hemoglobin


Enzyme Inhibition


Multisubstrate Kinetics


Fluorescence and Denaturation


Fluorescence Studies of Ligand Binding


DNA Restriction Digests


Western Blotting

Om forfatteren

Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.