Microwave Techniques and Protocols

Richard T. Giberson (Redaktør) ; Richard S. Demaree Jr. (Redaktør)

The contributors to this manual are uniformly from those labora- ries routinely using microwave technology to facilitate their processing methods in the various fields of microscopy. The methods and results these authors describe are the tangible evidence that microwaves can be used routinely as the basis for improved sample processing for micr- copy applications. Les mer
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The contributors to this manual are uniformly from those labora- ries routinely using microwave technology to facilitate their processing methods in the various fields of microscopy. The methods and results these authors describe are the tangible evidence that microwaves can be used routinely as the basis for improved sample processing for micr- copy applications. These applications include complete sample proce- ing protocols for light and electron microscopy, decalcification, and immunocytochemistry. The overall time savings, ease of use, and qu- ity of results serve as justification for using microwaves in the labo- tory. The question as to whether there is a "microwave effect" is alluded to, but not discussed in any great detail. When the term microwave technology is used, it is generic and intended to mean equipment designed for laboratory versus household use. Microwave Techniques and Protocols is designed for anyone with a background and experience in sample processing for immunocytoch- istry, decalcification, light microscopy, or electron microscopy, and clearly demonstrates that microwave technology has a place in today's laboratory. Richard T. Giberson, Richard S.
Demaree Jr. , REFERENCES Giberson, R. T. Demaree, R. S. , and (1995) Microwave fixation: understa- ing the variables to achieve rapid reproducible results. Microsc Res Tech 32:246-254. Giberson, R. T. , Demaree, R. S. , Jr. , and Nordhausen, R. W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61-67. Jr. VhD Ms vi Preface ...v Contributors ...

Fakta

Innholdsfortegnelse

Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy
Richard S. Demaree, Jr. and Richard T. Giberson

Vacuum-Assisted Microwave Processing of Animal Tissues for Electron Microscopy
Richard T. Giberson

Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy
William A. Russin and Christina L. Trivett

Basic Procedure for Electron Microscopy Processing and Staining in Clinical Laboratory Using Microwave Oven
Ronald L. Austin

Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnostic Laboratory
Robert W. Nordhausen and Bradd C. Barr

Microwave Processing of Archived Pathology Specimens for Ultrastructural Examination
Robert J. Munn and Phillip J. Vogt

Microwave Fixation of Rat Hippocampal Slices
Marcia D. Feinberg, Karen M. Szumowski, and Kristen M. Harris

Microwave Processing Techniques for Biological Samples in a Service Laboratory
Lou Ann Miller

Microwave-Accelerated Decalcification: Useful Methods for Research and Clinical Laboratories
Victoria J. Madden

Microwave Processing of Sediment Samples
Dawn Lavoie, Janet Watkins, and Yoko Furukawa

Microwave Polymerization in Thin Layers of London Resin White Allows Selection of Specimens for Immunogold Labeling
Jennifer E. Lonsdale, Kent L. McDonald, and Russell L. Jones

In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei
Mark A. Sanders and David M. Gartner

Microwave-Assisted Cytochemistry: Accelerated Visualization of Acetylcholinesterase at Motor Endplates
John P. Petrali and Kenneth R. Mills

Microwave-Assisted Immunoelectron Microscopy of Skin: Localization of Laminin, Type IV Collagen, and Bullous Pemphigoid Antigen
John P. Petrali and Kenneth R. Mills

Microwave Paraffin Techniques for Botanical Tissues
Denise Schichnes, Jeffrey A. Nemson, and Steven E. Ruzin

Microwave-Assisted Formalin Fixation of Fresh Tissue: A Comparative Study
Richard T. Giberson and Douglas E. Elliott

Microwave-Assisted Processing of Biological Samples for Scanning Electron Microscopy
Richard S. Demaree, Jr