Laboratory Protocols in Fungal Biology
Current Methods in Fungal Biology
Vijai Kumar Gupta (Redaktør) ; Maria Tuohy (Redaktør)
Mycology has an integral role to play in the development of the biotechnology and biomedical sectors. It has become a subject
of increasing importance as new fungi and their associated biomolecules are identified. Les mer
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Mycology has an integral role to play in the development of the biotechnology and biomedical sectors. It has become a subject
of increasing importance as new fungi and their associated biomolecules are identified. As this discipline comes to the forefront
of research in these sectors, the requirement for a consolidation of available research approaches is required. The First
Edition of this book has a few basic and applied protocols. With the Second Edition, this book provides consolidated information
on recent developments and the most widely used mycological methods available in the fields of biochemistry, biotechnology
and microbiology. The methods outlined offer clear and concise directions to the reader and covers both standard protocols
and more applied mycological methods. This book provides useful information for undergraduates, post-graduates, and specialists
and researchers studying fungal biology.
- FAKTA
-
Utgitt:
2022
Forlag: Springer Nature Switzerland AG
Innbinding: Innbundet
Språk: Engelsk
Sider: 261
ISBN: 9783030837488
Utgave: 2. utg.
Format: 24 x 16 cm
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Preface.
Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results
Dr. Svetlana M. Ozerskaya
All-Russian Collection of Microorganisms (VKM IBPM RAS, Pushchino, Russia), G.K.Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, prospekt Nauki, 5, Pushchino-142290, Moscow Region, Russia.
Phone: +7-499-783-25-02, Fax: +7-499-783-25-02
Email: smo@dol.ru
2. Confocal Laser and Epifluorescence microscopy
Prof. Marcelo de Carvalho Alves
Department of Soil and Rural Engineering, Federal University of Mato Grosso, Brazil.
E-mail: mdecalves@ufmt.br; marcelocarvalhoalves@gmail.com
3. Diagnose of chytid parasites of algae in the plankton
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr
4. Sabouraud Agar for Fungal Growth
Dr. Janelle Hare
Department of Biology & Chemistry, Morehead State University , Morehead, KY, USA.
Phone: +606-783-2951, Fax: +606-783-5002
Email: jm.hare@moreheadstate.edu
5. FISH, fluorescence in situ hybridization of uncultured zoosporic fungi
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr, latestuggine@gmail.com
6. Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi
Dr. Jose M. Diaz-Minguez
Prof. Titular de Universidad, Director Centro Hispano Luso de Investigaciones Agrarias,CIALE, Universidad de Salamanca, C/ Rio Duero, 12, Campus de Villamayor, 37185 Salamanca, Spain.
Phone: 34 923 294500 ext. 5101, Fax 34 923 294399
E-mail: josediaz@usal.es
7. Real-time quantitative PCR assay for counting uncultured zoosporic fungi
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr 8. Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes
Dr. C.K.M. Tsui
Department of Forest Sciences, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Phone: +1 604 822 3108,Fax: +1 604 822 9102
Email: clementsui@gmail.com
9. Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi
Dr. M. Pandi
1Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-625 021, Tamilnadu, India.
Phone: +91-9840652927, Fax: +91-0452-2459105
Email: an_pandi@rediffmail.com
10. Recent advances in applications of Machine Learning in Fungal Biology
Dr. V. K. Jayaraman
Scientific and Engineering computing Group (SECG), Centre for Development of Advanced Computing (C-DAC), Ganeshkhind, Pune-411007, Maharashtra, India.
Phone: +91-20-2570-4100, Fax: +91-20-2569 4004
Email: jayaramanv@cdac.in
11. TBD (on Fungal Conservation)
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr
12. TBD
Professor Hauke Smidt, Wageningen University & Research, Laboratory of Microbiology, Stippeneng 4, 6708 WE Wageningen, Netherlands,
Email: hauke.smidt@wur.nl
13 Loop-mediated isothermal amplification of the entomopathogenic fungi, Beauveria bassiana
Dr Stefan T. Jaronski
Research Entomologist
USDA ARS Northern Plains Agricultural Research Laboratory
1500 N. Central Ave.
Sidney MT 59270 USA
Tel. 406-433-9486
Email: Stefan.Jaronski@ars.usda.gov
14. Molecular taxonomy and multi-gene phylogeny of fungi
Dr. Abhishek Baghela
Scientist - D,
National Fungal Culture Collection of India (NFCCI)
Biodiversity & Palaeobiology Group
MACS - Agharkar Research Institute
G. G. Agarkar Road, Pune 411004, India
(Lab)+91 20 25325105
(M) +91 7057180433
Email: abhishekbaghela@aripune.org
15. Yeast isolation methods from specialized habitats
Dr. Abhishek Baghela
Scientist - D,
National Fungal Culture Collection of India (NFCCI)
Biodiversity & Palaeobiology Group
MACS - Agharkar Research Institute
G. G. Agarkar Road, Pune 411004, India
(Lab)+91 20 25325105
(M) +91 7057180433
Email: abhishekbaghela@aripune.org
16. Assays for the quantification of antioxidant enzymes in fungi
Professor Konstantinos Grintzalis,
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Universite Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium.
Email: kgrintzalis@gmail.com.
Index.
Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results
Dr. Svetlana M. Ozerskaya
All-Russian Collection of Microorganisms (VKM IBPM RAS, Pushchino, Russia), G.K.Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, prospekt Nauki, 5, Pushchino-142290, Moscow Region, Russia.
Phone: +7-499-783-25-02, Fax: +7-499-783-25-02
Email: smo@dol.ru
2. Confocal Laser and Epifluorescence microscopy
Prof. Marcelo de Carvalho Alves
Department of Soil and Rural Engineering, Federal University of Mato Grosso, Brazil.
E-mail: mdecalves@ufmt.br; marcelocarvalhoalves@gmail.com
3. Diagnose of chytid parasites of algae in the plankton
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr
4. Sabouraud Agar for Fungal Growth
Dr. Janelle Hare
Department of Biology & Chemistry, Morehead State University , Morehead, KY, USA.
Phone: +606-783-2951, Fax: +606-783-5002
Email: jm.hare@moreheadstate.edu
5. FISH, fluorescence in situ hybridization of uncultured zoosporic fungi
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr, latestuggine@gmail.com
6. Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi
Dr. Jose M. Diaz-Minguez
Prof. Titular de Universidad, Director Centro Hispano Luso de Investigaciones Agrarias,CIALE, Universidad de Salamanca, C/ Rio Duero, 12, Campus de Villamayor, 37185 Salamanca, Spain.
Phone: 34 923 294500 ext. 5101, Fax 34 923 294399
E-mail: josediaz@usal.es
7. Real-time quantitative PCR assay for counting uncultured zoosporic fungi
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr 8. Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes
Dr. C.K.M. Tsui
Department of Forest Sciences, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Phone: +1 604 822 3108,Fax: +1 604 822 9102
Email: clementsui@gmail.com
9. Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi
Dr. M. Pandi
1Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-625 021, Tamilnadu, India.
Phone: +91-9840652927, Fax: +91-0452-2459105
Email: an_pandi@rediffmail.com
10. Recent advances in applications of Machine Learning in Fungal Biology
Dr. V. K. Jayaraman
Scientific and Engineering computing Group (SECG), Centre for Development of Advanced Computing (C-DAC), Ganeshkhind, Pune-411007, Maharashtra, India.
Phone: +91-20-2570-4100, Fax: +91-20-2569 4004
Email: jayaramanv@cdac.in
11. TBD (on Fungal Conservation)
Prof. Telesphore Sime-Ngando
LMGE, Laboratoire Microorganismes, Genome et Environnement, UMR CNRS 6023, Universite Blaise Pascal, Clermont Universite, BP 80026, 63171 Aubiere Cedex, France.
Phone : +33473407836, Fax: +33473407670
Email: telesphore.sime-ngando@univ-bpclermont.fr
12. TBD
Professor Hauke Smidt, Wageningen University & Research, Laboratory of Microbiology, Stippeneng 4, 6708 WE Wageningen, Netherlands,
Email: hauke.smidt@wur.nl
13 Loop-mediated isothermal amplification of the entomopathogenic fungi, Beauveria bassiana
Dr Stefan T. Jaronski
Research Entomologist
USDA ARS Northern Plains Agricultural Research Laboratory
1500 N. Central Ave.
Sidney MT 59270 USA
Tel. 406-433-9486
Email: Stefan.Jaronski@ars.usda.gov
14. Molecular taxonomy and multi-gene phylogeny of fungi
Dr. Abhishek Baghela
Scientist - D,
National Fungal Culture Collection of India (NFCCI)
Biodiversity & Palaeobiology Group
MACS - Agharkar Research Institute
G. G. Agarkar Road, Pune 411004, India
(Lab)+91 20 25325105
(M) +91 7057180433
Email: abhishekbaghela@aripune.org
15. Yeast isolation methods from specialized habitats
Dr. Abhishek Baghela
Scientist - D,
National Fungal Culture Collection of India (NFCCI)
Biodiversity & Palaeobiology Group
MACS - Agharkar Research Institute
G. G. Agarkar Road, Pune 411004, India
(Lab)+91 20 25325105
(M) +91 7057180433
Email: abhishekbaghela@aripune.org
16. Assays for the quantification of antioxidant enzymes in fungi
Professor Konstantinos Grintzalis,
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Universite Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium.
Email: kgrintzalis@gmail.com.
Index.
Vijai Kumar Gupta is currently working as Senior Fellow at Center for Safe and Improved Foods & Biorefining and Advanced Materials
Research Center, Scotland's Rural College (SRUC), Edinburgh, UK. Dr Gupta received his doctoral degree in Microbiology in
2009 from Dr RML Avadh University India, and the leading expert in the area of Biomass Valorisation, Microbial Bioprocess
Biotechnologies, Bioactive Natural Products, and Environmental Sustainability.
He is the Secretary of the European Mycological Association and also the International Professor at IFST-China, Visiting Professor at SCBG-China; SZIU-Hungary; SHUATS-India. Dr Gupta is the Fellow of few international scientific societies including- Linnaean Society-UK; Mycological Society of India; Indian Mycological Association; Academy of Microbiological Sciences India; National Academy of Agricultural Sciences, India and Member of Royal Society Biology, UK. He has 175 peer-reviewed publications, 33 book chapters and 39 books in his hands with author h-index 50, i10-index 173 with total 11K+ citations to-date. Also, he is the editor of few reputed journals including, Microbial Cell Factories, Scientific Reports, Microbiome, Biotechnology Advances and Critical Reviews in Biotechnology.
Maria Tuohy is the Principal Investigator and Head of the Molecular Glycobiotechnology Group, School of Natural Sciences, NUI, Galway, Ireland. Dr. Tuohy has a strong track record in Glycobiotechnology and Enzyme Biotechnology, with ~28 years' experience in fungal biology and biotechnology. A special focus of the research is on developing enabling technology for bioconversion of terrestrial plants and algae for biorefinery and bioenergy applications. This research encompasses protein biotechnology, molecular enzymology, proteomics, fungal genetics, biology and genomics, fermentation, analytical methods in glycobiology and protein biochemistry, development of novel energy systems, as well as key analytical methods to measure and characterize novel bioactive compounds from microbial, plant and marine sources. Understanding fungal biodiversity and the relationships between fungi and other organisms (terrestrial plants and marine life) forms a central theme that underpins the research on developing fungi as cell factories for production of enzymes and bioactives of biotechnological relevance. Research to date has involved ongoing collaboration with industry. Dr. Tuohy is one of 5 PIs in the EI-IDA supported, industry-led, national Technology Centre for Biorefining and Bioenergy (TCBB) and more recently, TCBB Resource. Through TCBB is involved in the European Bio- based Industries Consortium (BIC) and was a PI on the Technological Innovation work package of an EU FP7 INTERREG IVB NWE Programme ('ReNEW'), which was coordinated by The Queens University Belfast and involved a consortium of RTD, Industry and regional development organizations across NW Europe. Dr. Tuohy is also a co-PI on Pillar 5 of the recent national Dairy Processing Technology Centre (DPTC; EURO25m funding), since February 2015, and a collaborating PI in the recent SFI- Industry supported 'Sustainable Energy & Fuel Efficiency Spoke' in MaREI Research Centre. Dr. Tuohy & her group are members of the Energy Research Centre and Environmental Technologies PRA group, Ryan Institute, NUI Galway, Ireland. Dr. Tuohy has successfully mentored 33 PhD students to completion to-date, and was awarded a prestigious President's Award for Teaching Excellence in 2011-2012 by NUI Galway, Ireland.
He is the Secretary of the European Mycological Association and also the International Professor at IFST-China, Visiting Professor at SCBG-China; SZIU-Hungary; SHUATS-India. Dr Gupta is the Fellow of few international scientific societies including- Linnaean Society-UK; Mycological Society of India; Indian Mycological Association; Academy of Microbiological Sciences India; National Academy of Agricultural Sciences, India and Member of Royal Society Biology, UK. He has 175 peer-reviewed publications, 33 book chapters and 39 books in his hands with author h-index 50, i10-index 173 with total 11K+ citations to-date. Also, he is the editor of few reputed journals including, Microbial Cell Factories, Scientific Reports, Microbiome, Biotechnology Advances and Critical Reviews in Biotechnology.
Maria Tuohy is the Principal Investigator and Head of the Molecular Glycobiotechnology Group, School of Natural Sciences, NUI, Galway, Ireland. Dr. Tuohy has a strong track record in Glycobiotechnology and Enzyme Biotechnology, with ~28 years' experience in fungal biology and biotechnology. A special focus of the research is on developing enabling technology for bioconversion of terrestrial plants and algae for biorefinery and bioenergy applications. This research encompasses protein biotechnology, molecular enzymology, proteomics, fungal genetics, biology and genomics, fermentation, analytical methods in glycobiology and protein biochemistry, development of novel energy systems, as well as key analytical methods to measure and characterize novel bioactive compounds from microbial, plant and marine sources. Understanding fungal biodiversity and the relationships between fungi and other organisms (terrestrial plants and marine life) forms a central theme that underpins the research on developing fungi as cell factories for production of enzymes and bioactives of biotechnological relevance. Research to date has involved ongoing collaboration with industry. Dr. Tuohy is one of 5 PIs in the EI-IDA supported, industry-led, national Technology Centre for Biorefining and Bioenergy (TCBB) and more recently, TCBB Resource. Through TCBB is involved in the European Bio- based Industries Consortium (BIC) and was a PI on the Technological Innovation work package of an EU FP7 INTERREG IVB NWE Programme ('ReNEW'), which was coordinated by The Queens University Belfast and involved a consortium of RTD, Industry and regional development organizations across NW Europe. Dr. Tuohy is also a co-PI on Pillar 5 of the recent national Dairy Processing Technology Centre (DPTC; EURO25m funding), since February 2015, and a collaborating PI in the recent SFI- Industry supported 'Sustainable Energy & Fuel Efficiency Spoke' in MaREI Research Centre. Dr. Tuohy & her group are members of the Energy Research Centre and Environmental Technologies PRA group, Ryan Institute, NUI Galway, Ireland. Dr. Tuohy has successfully mentored 33 PhD students to completion to-date, and was awarded a prestigious President's Award for Teaching Excellence in 2011-2012 by NUI Galway, Ireland.