Synthetic Biology

Parts, Devices and Applications

; Jens Nielsen ; Gregory Stephanopoulos ; Christina Smolke (Redaktør)

A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering.

Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. Les mer
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A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering.

Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy.

Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.

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Innholdsfortegnelse

A review of the interdisciplinary field of synthetic biology, from genome design to spatial Engineering.


Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy.


Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.


A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering.






Written by an international panel of experts,
Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy.






Filled with the most recent research, compelling discussions, and unique perspectives,
Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.

About the Series Editors xv


Part I DNA Synthesis and Genome Engineering 1


1 Competition and the Future of Reading and Writing DNA 3
Robert Carlson


1.1 Productivity Improvements in Biological Technologies 3


1.2 The Origin of Moore s Law and Its Implications for Biological Technologies 5


1.3 Lessons from Other Technologies 6


1.4 Pricing Improvements in Biological Technologies 7


1.5 Prospects for New Assembly Technologies 8


1.6 Beyond Programming Genetic Instruction Sets 10


1.7 Future Prospects 10


References 11


2 Trackable Multiplex Recombineering (TRMR) and Next-Generation Genome Design Technologies: Modifying Gene Expression in E. coli by Inserting Synthetic DNA Cassettes and Molecular Barcodes 15
Emily F. Freed, Gur Pines, Carrie A. Eckert, and Ryan T. Gill


2.1 Introduction 15


2.2 Current Recombineering Techniques 16


2.2.1 Recombineering Systems 17


2.2.2 Current Model of Recombination 17


2.3 Trackable Multiplex Recombineering 19


2.3.1 TRMR and T2RMR Library Design and Construction 19


2.3.2 Experimental Procedure 23


2.3.3 Analysis of Results 24


2.4 Current Challenges 25


2.4.1 TRMR and T2RMR are Currently Not Recursive 26


2.4.2 Need for More Predictable Models 26


2.5 Complementing Technologies 27


2.5.1 MAGE 27


2.5.2 CREATE 27


2.6 Conclusions 28


Definitions 28


References 29


3 Site-Directed Genome Modification with Engineered Zinc Finger Proteins 33
Lauren E. Woodard, Daniel L. Galvan, and Matthew H. Wilson


3.1 Introduction to Zinc Finger DNA-Binding Domains and Cellular Repair Mechanisms 33


3.1.1 Zinc Finger Proteins 33


3.1.2 Homologous Recombination 34


3.1.3 Non-homologous End Joining 35


3.2 Approaches for Engineering or Acquiring Zinc Finger Proteins 36


3.2.1 Modular Assembly 37


3.2.2 OPEN and CoDA Selection Systems 37


3.2.3 Purchase via Commercial Avenues 38


3.3 Genome Modification with Zinc Finger Nucleases 38


3.4 Validating Zinc Finger Nuclease-Induced Genome Alterati

Om forfatteren

Sang Yup Lee is Distinguished Professor at the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST).

Jens Nielsen is Professor and Director to Chalmers University of Technology, Sweden. He has received numerous Danish and international awards including the Nature Mentor Award.

Professor Gregory Stephanopoulos is the W. H. Dow Professor of Chemical Engineering at the Massachusetts Institute of Technology and Director of the MIT Metabolic Engineering Laboratory.