Cell-derived Matrices Part A

David Caballero (Redaktør) ; Subhas C. Kundu (Redaktør) ; Rui L Reis (Redaktør)

Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Les mer
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Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific chapters cover Anisotropic cell-derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for bioprinting applications, and much more.

Fakta

Innholdsfortegnelse

Preface David Caballero, Subhas C Kundu and Rui L Reis

Section 1: Decellularization and characterization methods

1.A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix scaffolds Dhavan Sharma, Morgan Ferguson and Feng Zhao

2.Preparation of osteogenic matrices from cultured cells Carl A. Gregory, Eoin McNeill and Simin Pan

3.Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches Fátima Raquel Maia, Rui Luís Reis and Joaquim Miguel Oiveira

4.Characterization of the elastic properties of extracellular matrix models by atomic force microscopy J. Otero, D. Navajas and J. Alcaraz

5.Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M. S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth and Robert J. Linhardt

Section 2: Engineering cell-derived matrices with advanced characteristics

6.Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices Janusz Franco-Barraza, Kristopher S. Raghavan, Tiffany Luong and Edna Cukierman

7.Engineering cell-derived matrices with controlled 3D architectures for pathophysiological studies Enrico Almici, David Caballero, Joan Montero Boronat and Josep Samitier Martí

8.Generation of fluorescent cell-derived-matrix to study 3D cell migration Amélie Luise Godeau, Hélène Delanoë-Ayari and Daniel Riveline

9.Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM Insung Yong, Seung Won Oh and Pilnam Kim

Section 3: Cell-derived matrices for biomedical applications

10.Cell-derived matrices (CDM)—Methods, challenges and applications Cheng HW, Yuan MT, Li CW and Chan BP.

11.Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail Tamara Rosell-García and Fernando Rodríguez-Pascual

12.Generating specific cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies Felix Yemanyi, Janice Vranka and VijayKrishna Raghunathan

13.Methods in cell biology: Cell-derived matrices Elizabeth Pavez Loriè and Petra Boukamp

Om forfatteren

Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications. Prof. Subhas C. Kundu is a European Research Area Chair and Professor at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho (Portugal). Earlier Prof. Kundu was a Professor in the Department of Biotechnology at the Indian Institute of Technology Kharagpur (India). He obtained his post-graduation and PhD in Genetics from Banaras Hindu University (India) and received his post-doctoral trainings at the Institute of Molecular Biology in Moscow, the York University in Canada, the Medical University in Lubeck (Germany), and the Brunel University in UK. His research interests include silk biomaterial matrices for biomedical applications including 3D cancer modelling for investigating tumor growth and progression. His present area of interest is the use of natural-based biomaterials for 3D cancer modelling and drug screening. Professor Rui L. Reis, PhD, DSc, Hon. Causa MD, Hon Causa PhD, FBSE, FTERM. He’s Vice-President for Research and Innovation of University of Minho, Portugal, Director of 3B’s Research Group and Director of ICVS/3B´s Associate Laboratory, both of UMinho. He’s the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, the Coordinator of the Discoveries Centre for Regenerative and Precision Medicine, the Global Past-President of the Tissue Engineering and Regenerative Medicine International Society and the Editor-in-chief of the Journal of Tissue Engineering and Regenerative Medicine. He’s edited several books and has more 1225 published works listed on ISI Web of Knowledge, being an inventor of around 70 patents. He’s been awarded many important international prizes, including the TERMIS-EU contributions to the literature Award and the TERMIS-EU Career Achievement Award, and recently the UNESCO- International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize.