Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Matthieu Piel (Redaktør) ; Junsang Doh (Redaktør) ; Daniel Fletcher (Redaktør)

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Les mer
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Om boka

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration.

Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more.

Fakta

Innholdsfortegnelse

Section 1 - Microfluidics for micro-organisms 1. Temperature control and drug delivery for cell division cycle control in fission yeast 2. H2O2 stress response in budding yeast 3. Antibiotic resistance in bacteria 4. Metabolism in bacteria 5. Fluidized beds for bacterial sorting and amplification

Section 2 - Microfluidics for cell culture and cell sorting of mammalian cells 6. Hydrogel microwells 7. Cell sorting with conical filters 8. Directed neuronal cell culture

Section 3 - Microfluidics for cell migration 9. Immune cells migration in complexe environments 10. Neutrophiles migration in health and disease 11. Cell guidance by physical cues 12. Stable gradients in gels of extracellular matrix for cancer cell migration

Section 4 - Microfluidics for cell mechanics 13. Size based cell sorting and single cell mechanobiology 14. High throughput measure of single cell deformability/stiffness 15. Host-microbe interactions 16. Cell confinement and intracellular crowding

Section 5 - Microfluidics for Single cell analytics 17. Quantification of single cell secretion 18. Mechanical cell disruption

Om forfatteren

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development. Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh’s group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy. Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.