Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Matthieu Piel (Redaktør) ; Daniel Fletcher (Redaktør) ; Junsang Doh (Redaktør)

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Les mer
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Leveringstid: Sendes innen 21 dager

Om boka

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more.

Fakta

Innholdsfortegnelse

Section 1 Microfluidics for cell culture, cell manipulation and cell sorting 1. Viable cell culture in PDMS-based microfluidic devices Melikhan Tanyeri and Savas Tay 2. High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis Xuan Li and Abraham P. Lee 3. Artificial niche microarrays for identifying extrinsic cell-fate determinants Samy Gobaa, Raphael V. Gayet and Matthias P. Lutolf 4. Reconstruction of directed neuronal networks in a microfluidic device with asymmetric microchannels Josquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy, Jean-Michel Peyrin and Catherine Villard 5. Single-cell 3D electro-rotation Liang Huang, Peng Zhao, Fei Liang and Wenhui Wang

Section 2 Droplet-based microfluidics 6. Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assay Benjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi, Makiko Hatori and Adam R. Abate 7. Quantifying phenotypes in single cells using droplet microfluidics Fengjiao Lyu, Lucas R. Blauch and Sindy K.Y. Tang 8. Modular microfluidics for double emulsion formation Bryant Thompson, Nareh Movsesian, Christine Cheng, Prathamesh Karandikar, Malancha Gupta and Noah Malmstadt 9. Universal anchored-droplet device for cellular bioassays Gabriel Amselem, Sébastien Sart and Charles N. Baroud

Section 3 Microfluidics for cellular analysis 10. Cell biology at the interface of nanobiosensors and microfluidics Nikhil Bhalla, Hung-Ju Chiang and Amy Q. Shen 11. BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencing Arjun K. Aditham, Tyler C. Shimko and Polly M. Fordyce

Om forfatteren

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development. Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases. Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh’s group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy.