Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ
Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements
and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches.
The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have
greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since
the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers
and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry
for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward
protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.
Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians
and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived
from in situ hybridization and immunohistochemistry.