This technical paper on ornamental plant propagation in the tropics has been written to highlight the potential of tissue culture as an advanced propagation technology allowing for mass cloning of selected varieties or ecotypes. This document provides information on the state of the art of tissue culture propagation and gives technical details for propagating some 30 ornamental plant species. The aim of the paper is to enhance the technical capacity of public institutions and private entrepreneurs, thus leading to new production and market opportunities. It also aims to facilitate and encourage the use of techniques to promote ornamental plant diversity.