Correlative Light and Electron MIcroscopy

Thomas Muller-Reichert (Redaktør) ; Paul Verkade (Redaktør)

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. Les mer
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Innbundet
Legg i
Vår pris: 1991,-

(Innbundet) Fri frakt!
Leveringstid: Sendes innen 21 dager

Om boka

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Fakta

Innholdsfortegnelse

Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
Correlative Fluorescence and Transmission Electron Microscopy in Tissues
Correlative Light and Electron Microscopy in Parasite Research
Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
Correlative Light and Electron Microscopy of GFP
Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!
Capturing Endocytic Segregation Events with HPF-CLEM
Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
Visualizing Proteins in Electron Micrographs at Nanometer Resolution
Atmospheric Scanning Electron Microscope for Correlative Microscopy
Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

Om forfatteren

Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.

TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.
He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. His focus is on the study of sorting mechanisms in intracellular transport pathways and in the area of Synthetic Biology. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 85 papers and edited 4 books on CLEM (including 3 Volumes of the Methods in Cell Biology series).
PV obtained his PhD on electron microscopic studies of the peripheral nervous system at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001.
He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He has acted as chair and co-chair of the Electron Microscopy section of the Royal Microscopical Society and is closely involved with BioimagingUK shaping the UK imaging infrastructure landscape.
He has organised and taught on several courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. His lab is the home of the EMBO practical course on CLEM and he is Work Group leader for CLEM within the EU COST project COMULIS (COrrelated MUltimodal imaging in LIfe Sciences).